JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650417.1
Database:	nt	Go to original record
Description:	Eremophila pterocarpa voucher MELUD119117a plastid, partial genome.
Taxon:	Eremophila pterocarpa
Original size:	147632 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48325	48888	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttctatcg aaacttcaga
251   aaggatgaag gataagggta tatattgaat actatatcaa atgattaatg
301   atggccctaa tctgtatctg tattttttta tctgaaaaat ggaagaattg
351   gtatgaagtg attccacatt gaagaaagaa ttaaatattc cttcatcaaa
401   tcattcactc cacagtctga tagttctttt aaagaactga ttaatcggac
451   aagaataaag atagagtccc attctacatg tcaatattgg caacaatgca
501   atttatagta agaggaaaat ccgtcgactt ttaaagtcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details