JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650403.1
Database:	nt	Go to original record
Description:	Eremophila christophori voucher MELU pending plastid, complete genome.
Taxon:	Eremophila christophori
Original size:	142353 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48188	48757	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaamaa aggttcakaa
151   ascsaaaaaa aaaaaaaaan ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gcgttggtag aggaatcttt ccatcgaaac
251   ttcagaaagg atgaaggata aaggtatata ttgaatacta tatcaaatga
301   ttaatgatgg ccccaatctg tatctgtatt tttttatatg aaaaatggaa
351   gaattggtat gaagtgattc cacattgaag aaagaattaa atattccttc
401   atcaaatcat tcactccaca gtctgatagt tcttttaaag aactgattaa
451   tcggacaaga ataaagatag agtcccattc tacatgtcaa tattggcaac
501   aatgcaattt atagtaagag gaaaatccgt cgacttttaa agtcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details