JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650397.1
Database:	nt	Go to original record
Description:	Eremophila prolata voucher MELUD119055a plastid, partial genome.
Taxon:	Eremophila prolata
Original size:	151834 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48284	48850	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   agnnnngann nasgataaag gtatatattg aatactatat caaatgatta
301   atgatggccc taatytgtat ctgtattttt ttatatgaaa aatggaagaa
351   ttggtatgaa gtgattccac attgaagaaa gaatcaaata ttccttyatc
401   aaatcattca ctccacagtc tgatagttct tttaaagaac tgattaatyg
451   gacaagaata aagatagagt cccattctac atgtcaatat tggcaacaat
501   gcaatttata gtaagaggaa aatccgtcga cttttaaagt cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details