JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650382.1
Database:	nt	Go to original record
Description:	Eremophila macdonnellii voucher MELU pending plastid, complete genome.
Taxon:	Eremophila macdonnellii
Original size:	151307 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48098	48662	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   taaatggagt tgactgcgtt ggtagaggaa tctttccatc gaaacttcag
251   aaaggatgaa ggataaaggt atatattgaa tactatatca aatgattaat
301   gatggcccta atctgtatct gtattttttt atatgaaaaa tggaagaatt
351   ggtatgaagt gattccacat tgaagaaaga attaactatt ccttcatcaa
401   atcattcact ccacagtctg atagttcttt taaagaactg attaatcgga
451   caagaataaa gatagagtcc cattctacat gtcaatattg gcaacaatgc
501   aatttatagt aagaggaaaa tccgtcgact ttaaaagtcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details