JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650366.1
Database:	nt	Go to original record
Description:	Eremophila spongiocarpa voucher MELU pending plastid, complete genome.
Taxon:	Eremophila spongiocarpa
Original size:	151687 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48237	48809	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggataaagg tatatattga atactatatc aaatgattaa tgatatcaaa
301   tgattaatga tggccctaat ctgtatctgc atttttttat atgaaaaatg
351   gaagaattgg tatgaagtga ttccacattg aagaaagaat caaatattcc
401   ttcatcaaat cattcactcc acagtctgat agttctttta aagaactgat
451   taatcggaca agaataaaga tagagtccca ttctacatgt caatattggc
501   aacaatgcaa tttatagtaa gaggaaaatc cgtcgacttt taaagtcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details