JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650349.1
Database:	nt	Go to original record
Description:	Eremophila hillii voucher MELU pending plastid, complete genome.
Taxon:	Eremophila hillii
Original size:	151501 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48219	48776	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggatgaag gataaaggta tatattgaat actatatcaa atgattaatg
301   atggccctaa tctgtatttt tttatatgaa aaatggaaga attggtatga
351   agtgattcca cattgaagaa agaatcaaat attccttcat caaatcattc
401   actccacagt ctgatagttc ttttaaagaa ctgattaatc ggacaagaat
451   aaagatagag tcccattcta catgtcaata ttggcaacaa tgcaatttat
501   agtaagagga aaatccgtcg actttaaaag tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details