JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650328.1
Database:	nt	Go to original record
Description:	Eremophila cuneifolia voucher MELUD119266a plastid, complete genome.
Taxon:	Eremophila cuneifolia
Original size:	151728 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48242	48819	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   agaaaggatg aaggatagat gaaggataaa ggtatatatt gaatactata
301   tcaaatgatt aatgatggcc ctaatctgta tctgtatttt tttatatgaa
351   aaatggaaga attggtatga agtgattcca cattgaagaa agaatcaaat
401   attccttcat caaatcattc actccacagt ctgatagttc ttttaaagaa
451   ctgattaatc ggacaagaat aaagatagag tcccattcta catgtcaata
501   ttggcaacaa tgcaatttat agtaagagga aaatccgtcg acttttaaag
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details