JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650308.1
Database:	nt	Go to original record
Description:	Eremophila longifolia voucher MELUD119241a plastid, complete genome.
Taxon:	Eremophila longifolia
Original size:	151833 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48233	48791	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactgcgt tggtagagga atctttccat cgaaacttca
251   gaaaggataa aggtatatat tgaatactat atcaaatgat taatgatggc
301   cccaatctgt atctgtattt ttttatatga aaaatggaag aattggtatg
351   aagtgattcc acattgaaga aagaattaaa tattccttca tcaaatcatt
401   cactccacag tctgatagtt cttttaaaga actgattaat cggacaagaa
451   taaagataga gtcccattct acatgtcaat attggcaaca atgcaattta
501   tagtaagagg aaaatccgtc gacttttaaa gtcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details