JRC GMO-Amplicons | EURL GMFF

Record ID:	MT650304.1
Database:	nt	Go to original record
Description:	Leucophyllum zygophyllum voucher MELUD119237a plastid, partial genome.
Taxon:	Leucophyllum zygophyllum
Original size:	154160 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48432	48992	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaaggtatat attgaatact atatcaaatg attaatgatg
301   gccctaatct gtatctgtat ttttttatat gaaaaatgga agaattggta
351   tgaagtgatt ccacattgaa gaaagaatca aatattcctt catcaaatca
401   ttcactccac agtctgatag ttcttttaaa gaactgatta atcggacaag
451   aataaagata gagtcccatt ctacatgtca atattggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aagtcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details