JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ564499.1
Database:	nt	Go to original record
Description:	Veronica arvensis chloroplast, complete genome.
Taxon:	Veronica arvensis
Original size:	149381 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46327	46901	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctatctt ctaaaaacaa aggttcagga
151   agtgaaaaaa ggggataggt gcagagactc gatggaagct gttctaacaa
201   atggagttga ctgtgtcagt agatgaatct tttttttttg aaacttcaga
251   aaggatgaag gataaacgga tctattgaat actatattat ataaaaaaag
301   gaacggtggg ccaaatctgt atctgtattt tgtttagatg caaaagaaaa
351   aaaaaaagac ttggtgtaaa gtaattccac attcaagaaa gaatcgaata
401   ttcatcaaat ccaccccact ccatagtctg atagatcttt taaagaattg
451   attaatcgga tgagaataaa gatagagtcc cattctacat gtcaataccg
501   acaaaaagga aatttatagt aagaggaaaa tccgtcgaat ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details