JRC GMO-Amplicons | EURL GMFF

Record ID:	ON598343.1
Database:	nt	Go to original record
Description:	Silene noctiflora voucher Ge130376 chloroplast, complete genome.
Taxon:	Silene noctiflora
Original size:	151686 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	38073	38600	-	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt tccttttttc aaaagcaaaa
151   aaagtattaa gaaagcaaga aaaaaaggat aggtgcagag actcaaaggg
201   agttgttcta acaaatgggg ttgactgtgt tgttataagt ataagacttc
251   ttccctctaa attccaaacc aagaaaaagg gtgaagaata tacgtactaa
301   caagaattgt tgtgaatcga ttccaagtta aaagtggaat ccatatttat
351   tcatcaaaac attcacactc actccatagt ctgatagatc ttgtgaagaa
401   ctgattaatc ggatgagaat aaagatagag tcctgttcta catgtcaata
451   ctgacaacaa tgaaatttat agtaagagga aaatccgtcg acttttaaaa
501   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details