JRC GMO-Amplicons | EURL GMFF

Record ID:	ON598339.1
Database:	nt	Go to original record
Description:	Zygophyllum fabago voucher Ge130213 chloroplast, complete genome.
Taxon:	Zygophyllum fabago
Original size:	105439 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46940	47486	+	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattacaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaaaaa taattcaatc
151   aaaagaatca aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   aaaatggagt tgactgctgc attaagttag tcaaggaatc cttgcatcaa
251   aactttcaaa aagatgaagg ataacctttc tttctacttt ctataaacta
301   taaaaagaaa aagacttttt gaatcaaatc aattggaagt tgaagaaaga
351   atcaaatatg atttgatcaa atcatttact ccaaagtctg atatatcttt
401   ttcaaaatgg attagtcaga tgagaataaa gatagagtcc cattctatat
451   gtcagtattg acaacaatat tgacaacaat gaaatttata gtgagaggaa
501   aatccgtcga ttttataaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details