JRC GMO-Amplicons | EURL GMFF

Record ID:	ON598336.1
Database:	nt	Go to original record
Description:	Zygophyllum mucronatum voucher MQ149 chloroplast, complete genome.
Taxon:	Zygophyllum mucronatum
Original size:	104942 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47010	47552	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaaaaa agaattcaat
151   caaaagaatc aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   aaaaatggag ttgactgtgc attaagttag tcaagtcaag gaatccttgc
251   atcaaaactt tcaaaaagat gaaggataac ctttctttct ataaaactat
301   aaaaagaaaa agactttttg aattaattga atcaaatcaa ttggaagttg
351   aagaaagaat caaatatgat ttgatcaaat aatttactcc aaagtctgat
401   atatcttttt caaaatagat tagtcagatg agaataaaga tagagtccca
451   ttctatatgt cagtattgac aacaatgaaa tttatagtga gaggaaaatc
501   cgtcgatttt ataaatcgtg agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details