JRC GMO-Amplicons | EURL GMFF

Record ID:	ON598333.1
Database:	nt	Go to original record
Description:	Zygophyllum pterocarpum voucher Ge130304 chloroplast, complete genome.
Taxon:	Zygophyllum pterocarpum
Original size:	105411 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47089	47621	+	533 bp

Amplicon:

533 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaaaat aattcaatca
151   aaagaatcaa aaaaggatag gtgcagagac tcaatggaag ctgttctaaa
201   aaatggagtt gactgctgca ttaagttagt caagtcaagg aatccttgca
251   tcaaaacttt caaaaagatg aaggataacc tttctttcta taaaactata
301   aaaagaaaaa gactttttga atcaaatcaa ttggaagttg aagaaagaat
351   caaatatgat ttgatcaaat catttactcc aaagtctgat atatcttttt
401   caaaatggat tagtcagatg agaataaaga tagagtccca ttctatatgt
451   cagtattgac aacaatgaaa tttatagtga gaggaaaatc cgtcgatttt
501   ataaatcgtg agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	533	514	2.40e-08

Match details