JRC GMO-Amplicons | EURL GMFF

Record ID:	ON598330.1
Database:	nt	Go to original record
Description:	Zygophyllum rosowii voucher Q-200 chloroplast, complete genome.
Taxon:	Zygophyllum rosowii
Original size:	104563 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46411	46942	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtagggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaaata attcaatcaa
151   aagaatcaaa aaaggatagg tgcagagact caatggaagc tgttctaaaa
201   aatggagttg actgctgcat taagttagtc aagtcaagga atccttgcat
251   caaaactttc aaaaagatga aggataacct ttctttctat aaaactataa
301   aaagaaaaag actttttgaa tcaaatcaat tggaagttta agaaagaatc
351   aaatatgatt tgatcaaata atttactcca aagtctgata tatctttttc
401   aaaatagatt agtcagatga gaataaagat agagtcccat tctatatgtc
451   agtattgaca acaatgaaat ttatagtgag aggaaaatcc gtcgatttta
501   taaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details