JRC GMO-Amplicons | EURL GMFF

Record ID:	ON598328.1
Database:	nt	Go to original record
Description:	Zygophyllum xanthoxylon voucher TLF-128 chloroplast, complete genome.
Taxon:	Zygophyllum xanthoxylum
Original size:	105348 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46989	47520	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaagaa ttcaattaaa
151   agaatcaaaa aaggataggt gcagagactc aatggaagct gttctaaaaa
201   atggagttga ctgctgcatt acattagtca agtcaagtaa tccttgcatc
251   gaaattttca aaaagataaa ggataacctt tctttctata aaactataaa
301   aaaaaaagac tttatgaatc aattcgaagt tgaagaaaga atcaaatatg
351   atttgataaa atcatttact ccaaagtctg atatatcttt ttcaaaatgg
401   attagtcaga tgagaataaa gatagagtcc cattctatat gtcagtattt
451   acaattgaca acaatgaaat ttatagtgag aggaaaatcc gtcgatttta
501   taaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details