JRC GMO-Amplicons | EURL GMFF

Record ID:	OP262738.1
Database:	nt	Go to original record
Description:	Brandisia racemosa isolate 2 voucher KUN:ChenZ-007 plastid, complete genome.
Taxon:	Brandisia racemosa
Original size:	154833 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47544	48097	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg attgcgctgg tacaagaatg aaacttcaga aaggatgaag
251   gataaacgta tctattgaat actatatcaa aatggttgat taatgatggc
301   ccgaatctgt atctgtattt ttgaatacga aaaatggaag aattggtgtg
351   aattgattcc atattgaaga aagaatattc attcatcaaa tcattcactc
401   catagtccga tagatctttt aaagaactga ttaatcggac gagaataaag
451   atagagtccc attctacatg tcaataccgg caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details