JRC GMO-Amplicons | EURL GMFF

Record ID:	OP262715.1
Database:	nt	Go to original record
Description:	Brandisia glabrescens isolate LC 1 voucher KUN:ChenZ-004 plastid, complete genome.
Taxon:	Brandisia glabrescens
Original size:	155336 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47656	48210	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcccgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gattgcgctg gtagaggaat gaaacttcag aaaggatgaa
251   ggataaacgt atctattgaa tactatatca aaatggttga ttaatgatgg
301   cccgaatctg tatctgtatt tttgaatacg aaaaatggaa gaattggtgt
351   gaattgattc catattgaaa aaagaatatt cattcatcaa atcattcact
401   ccatagtccg atagatcttt taaagaactg attaatcgga cgagaataaa
451   gatagagtcc cattctacat gtcaataccg gcaacaatga aatttatagt
501   aagaggaaaa tccgtcgact tttaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details