JRC GMO-Amplicons | EURL GMFF

Record ID:	OP262709.1
Database:	nt	Go to original record
Description:	Brandisia discolor isolate L voucher KUN:LZ59 plastid, complete genome.
Taxon:	Brandisia discolor
Original size:	154915 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47679	48235	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaagggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgattgcgc tggtagagga atgaaacttc agaaaggatg
251   aaggataaac gtatctattg aatactatat caaaatggtt gattaatgat
301   ggcccgaatc tgtatctgta tttttgaata cgaaaaatgg aagaattggt
351   gtgaattgat tccatattga agaaagaata ttcattcatc aaatcattca
401   ctccatagtc cgatagatct tttaaagaac tgattaatcg gacgagaata
451   aagatagagt cccattctac atgtcaatac cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttcaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details