JRC GMO-Amplicons | EURL GMFF

Record ID:	OP262707.1
Database:	nt	Go to original record
Description:	Brandisia discolor isolate KP 1 voucher KUN:NWC-201937 plastid, complete genome.
Taxon:	Brandisia discolor
Original size:	154998 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47716	48271	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaagggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgattgcgct ggtagaggaa tgaaacttca gaaaggatga
251   aggataaacg tatctattga atactatatc aaaatggttg attaatgatg
301   gcccgaatct gtatctgtat ttttgaatac gaaaaatgga agaattggtg
351   tgaattgatt ccatattgaa gaaagaatat tcattcatca aatcattcac
401   tccatagtcc gatagatctt ttaaagaact gattaatcgg acgagaataa
451   agatagagtc ccattctaca tgtcaatacc ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttcaaaatc gtgagggttc aagtccctct
551   atcccc

View other 13 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details