Match details
Record ID: |
KT225497.1 |
Database: |
nt |
Go to original record |
Description: |
Oryza sativa Japonica Group cultivar Dong Jin sucrose phosphate synthase 3 gene, partial cds. |
Taxon: |
Oryza sativa Japonica Group |
Original size: |
1092 bp |
View other 1 match for this record
Method: |
QT-TAX-OS-003 |
Go to GMOMETHODS record |
Description: | Quantitative PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009). |
Target: | rice sucrose-phosphate synthase |
View other 14 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
63 |
142 |
+ |
80 bp |
|
1 ttgcgcctga acggatatct ttcagtttgt aaccccggat gacgcacgga
51 cggctcggat catcccgaaa agatcaaccg
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
18 bp |
0 |
0 |
1 |
18 |
4.42e-08 |
primerB |
100.0 |
20 bp |
0 |
0 |
80 |
61 |
3.94e-09 |
probe |
100.0 |
19 bp |
0 |
0 |
59 |
41 |
1.32e-08 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.