Match details

Record ID: KT225497.1
Database: nt Go to original record
Description: Oryza sativa Japonica Group cultivar Dong Jin sucrose phosphate synthase 3 gene, partial cds.
Taxon: Oryza sativa Japonica Group
Original size: 1092 bp

View other 1 match for this record


Method: QL-TAX-OS-006 Go to GMOMETHODS record
Description:Qualitative PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).
Target:rice sucrose-phosphate synthase

View other 10 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 63 339 + 277 bp

Amplicon: 277 bp
1     ttgcgcctga acggatatct ttcagtttgt aaccccggat gacgcacgga
51    cggctcggat catcccgaaa agatcaaccg cggcgcgagc acgagaccac
101   cgtgggcccc atggcccacc gacttacaca atctctccca ctgccatgcg
151   ggcccacacc cgcaacagtc cagtccagag agccccgaac tcctccaaac
201   ccgggggggc cacaccctgc cacgtgtcac ccgccggcct ccctctcatc
251   ctctctctcc tcgtccagtg cttctcc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 1.47e-07
primerB 100.0 19 bp 0 0 277 259 4.44e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.