JRC GMO-Amplicons | EURL GMFF

Record ID:	KM487701.1
Database:	nt	Go to original record
Description:	Chloranthus serratus tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Chloranthus serratus
Original size:	1010 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	570	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattttcgt aaagggttta gaaagcgaaa
151   ataatcaaaa ggataggtgc agagactcaa tggaagctgt tctaacgaat
201   ggagttgact gcgttgcgtt ggtagaggaa tccttctatc gaaattgcgg
251   aaaggcagaa aggatgatct tatatacgat acgtactaaa atatcaaatg
301   attaattaag acccgaatcc atattttttt atatgaaaaa tttcatagtt
351   cttgtgaatc gattcgaagt tgaaggaaga atcgaatatt cagtgatcaa
401   atcattcact tcagagtctg attctgatag atttttgaag aatttttgaa
451   tcagacgaga ataaagatag agtcccattc tacatgtcaa taccgacaac
501   aatgaaattt atagtaagag gaaaatccgt cgaccttaga aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details