JRC GMO-Amplicons | EURL GMFF

Record ID:	KM487699.1
Database:	nt	Go to original record
Description:	Sarcandra glabra tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Sarcandra glabra
Original size:	991 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	568	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctattttcat aaaacaaggg tttagaaagc
151   gaaaataatc aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatggagtt gactgcgttg cgttggtaga ggaatccttt tatcgaaatt
251   gcagaaaggc agaaaggata atcctatata cgtactaaaa tatcaaacga
301   ttaattaaga cccgaatcta tattttttat atgaaaaatt tcagagttct
351   tgtgaatcga ttccaagttg aaggaagaat cgaatattca gtgatcaaat
401   cattcacttc agagtctgat tctgatagat ttttgaagaa ttgattaatc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ccgacaacaa
501   tgaaatttat agtaagagga aaatccgtcg accttagaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details