JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965255.1
Database:	nt	Go to original record
Description:	Rutidea fuscescens isolate AG033 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Rutidea fuscescens
Original size:	1692 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	751	1320	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaacctgga attaataaaa
101   aggggcaatc ctgagccaaa tcccgttttc cgaaaccaaa ggttcagaaa
151   gtgaaaaggg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catctaaaat tccgaaaggg
251   taaagtgaaa gataaacgta tatatacgta ttgaatacta tatcaaacaa
301   atgattaatg acgactcgat tgaatctgta ttttttatat gaaaacggaa
351   gaattggtgt gaatagattc cacattgaag aaagaatcga atattcattg
401   atcaaatgat tcactccata gtctgataga tcttttcaag aattgattaa
451   tcggacaaga ataaagatag agtcctgttc tacatgtcaa tgtcggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details