JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965232.1
Database:	nt	Go to original record
Description:	Oligocodon cunliffeae isolate BR068 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Oligocodon cunliffeae
Original size:	1329 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	382	955	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcaagg
151   ttcagaaagt gaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttggctgcg ttagtagaga aatctttcca tctaaaattc
251   cgaaaggata aagtgaagga taaacgtata tatacgtatt gaatactata
301   tcaaatgatt aatgacgact cgactgaatc tatatttttt atatgaaaac
351   ggaagaattg gtgtgaatag attccacatt gaagaaggaa tcgaatattc
401   attgatcaaa tgattcactc catagtctga tagatctttt caagaattga
451   ttaatcggac gagaataaag atagagtccc gttctacatg tcaatgtcgg
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details