JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965187.1
Database:	nt	Go to original record
Description:	Cremaspora triflora isolate H097 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Cremaspora triflora
Original size:	1680 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	758	1322	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaacaaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatatgt attgaatact atatcaaatg
301   attaatgagg actcgactga atctgtattt tttatatgaa aacggaagaa
351   ttggtgtgaa tagattccac attgaaggaa gaatcgaata ttcattgatc
401   aaatgattca ctccatagtc tgatagatct tttcaagatt gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details