JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965185.1
Database:	nt	Go to original record
Description:	Cordiera triflora isolate BR064 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Cordiera triflora
Original size:	1734 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	771	1335	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    actcactaag tgataacttt caaattcagg gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag ataaatcttt ccatctaaaa ttctgaaagg
251   ataaagtgaa ggataaacgt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatatgaaaa cggaagaatt
351   ggtatgaata gattccacat tgaagaaaga atcgaatatt cattgatcaa
401   atgattgact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttataat aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details