JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965181.1
Database:	nt	Go to original record
Description:	Cladoceras subcapitatum isolate AG016 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Cladoceras subcapitatum
Original size:	1721 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	763	1329	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   gtaaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgacg actcgactga atccgtattt tttatatgaa aacggaagaa
351   ttggtgtgaa tagattacac attgaagaaa gaatcgaata ttcattgatc
401   aaatgattca ctccatagtc tgatagatct ttgcaagaat tgattaatcg
451   gacgagaata aagatagagt cctgttctac atgtcaatgt cggcaacaac
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details