JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965170.1
Database:	nt	Go to original record
Description:	Bungarimba ridsdalei isolate BY023 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Bungarimba ridsdalei
Original size:	1683 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	753	1327	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag gggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttggc tgcgttagta gagaaatctt tccatctaaa attccgaaag
251   gataaagtga aggataaacg tatatatacg tattgaatac tatatcaaat
301   gattaatgac gactcgactg aatctgtatt ttttattttt tatatgaaaa
351   cggaagaatt ggtgtgaata gattccacat tgaagaaaga atcgaatatt
401   cattgatcaa atgattcact ccatagtctg atagatcttt tcaagaattg
451   attaatcgga cgagaataaa gatagagtcc cgttctacat gtcaatgtcg
501   gcaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details