JRC GMO-Amplicons | EURL GMFF

Record ID:	KF965167.1
Database:	nt	Go to original record
Description:	Botryarrhena venezuelensis isolate BR062 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Botryarrhena venezuelensis
Original size:	1674 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	711	1277	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    actcactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaaa tcccgttttc cgaaaccaaa ggttcagaaa
151   gtgaaataag ggataggtgc agagactcaa cggaagctgt tctaacaaac
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgacg actcgactga atctgtattt tttatatgaa aacggaagaa
351   ttggtgtgaa tagattccac attgaagaaa gaatcgaata ttcattgatc
401   aaatgattca ctccatagtc tgatagatct tttcaagaat tgattaatcg
451   gacgagaata aagatagagt cccgttatac atgtcaatgt tggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details