JRC GMO-Amplicons | EURL GMFF

Record ID:	KP756701.1
Database:	nt	Go to original record
Description:	Cestrum elegans isolate R016 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cestrum elegans
Original size:	1741 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	755	1330	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctttatt tcggaaaaca aacaaaggtt
151   cagaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaaa ggaatcttta ctttacatcg aaacttcaga
251   aagaaaaaga atgaagtgaa ggataaacgt atatatatac gtattgaata
301   ctatatcaaa tgattaatga cgacccgaat ccatattttt tctataaaaa
351   atagaagaat tggtgtgaat cgattctaca ttgaagaaag aatcgaatat
401   tcattgatca aatcattcac tccatagtct gatagatctt ttgaagaact
451   gattaattgg acgagaataa agatagagtc ccgttctaca tgtcaatacc
501   ggcaacaatg aaatttatag taagaggaaa atccgtcgac tttaaaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details