JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ501271.1
Database:	nt	Go to original record
Description:	Telfairia pedata tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Telfairia pedata
Original size:	1026 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	600	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aagggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacgttgcg
201   ttagtaaagg aatgaatcct tccatcgaaa ctccagaaaa gatgaaagat
251   aaacgtatta cgtactgaaa tactatttga ttaatgacaa cccgaatctc
301   tattttttat atttttataa ttgttatata aaataacaaa tgatatataa
351   aaatgaacga attgttatga atcgattcca atatctccaa gttgaaaaaa
401   gaatcgaata ttcattgatt aaatcattta ctccatcata catagtctga
451   tagatctttt gaagaactga ttaatcagat gcgaatagaa taaagataga
501   gtcccatcct acatgtcaat accgacaaaa atgaaattta tagtaagagg
551   aaaatccgtc gactttcaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details