JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ501269.1
Database:	nt	Go to original record
Description:	Momordica charantia tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Momordica charantia
Original size:	1032 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	599	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaag gggggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacgttgcg
201   ttagtaaagg aatgaatgaa tccttccatc gaaactccag aaaagatgaa
251   agataaacgt attacgtact gaaatactat ttgattaatg acgacccgaa
301   tctatatttt ttatatttat atttttatat attttatata aaaatataaa
351   aatgaacgaa ttgttatgaa tcgattccaa tatctccaag ttgaaaaaag
401   aatcgaatat tcattgatca aatcatttac tccatcatac atagtctgat
451   agatcttttg aagaactgat taatcagatg cgaatagaat aaagatagag
501   tcccattcta catgtcaata ccgacaaaaa tgaaatttat agtaagagga
551   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details