JRC GMO-Amplicons | EURL GMFF

Record ID:	KR233284.1
Database:	nt	Go to original record
Description:	Trillium smallii tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Trillium smallii
Original size:	1043 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	595	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggcatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaag
101   gggcaatcct gagccaaatc tttcttttga gaaatcaagt gtttagtttc
151   taaactaaaa ctaaaaaaaa aaggataggt gcagagactc aatggaagct
201   gttctaacga atggagttga ctacgttgcg ttggtagctg gaatccctct
251   atcgaaatta aggagaggat ggccctatat atgtatacgt atatatactg
301   acatatcaaa cgattaatca cgacccgaat cgatatttga tattatatat
351   gaataaaatt cagaattatt gtgaatccat tccaatcgaa gttgaaggaa
401   gaatcacata ttcaatgatc aaataaatca ttcattccgg agtcttatat
451   atatatatat tttaaaactg attaatcgca cgagaataaa gagagagtcc
501   cattctacat gtcaataccg acaacaatga aatttatagt aagaggaaaa
551   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details