JRC GMO-Amplicons | EURL GMFF

Record ID:	OX459127.1
Database:	nt	Go to original record
Description:	Oldenlandia corymbosa var. corymbosa genome assembly, organelle: plastid
Taxon:	Oldenlandia corymbosa var. corymbosa
Original size:	152414 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47082	47664	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctaag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aaaacaaagg
151   ttcagaaagg gaaaaagggg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttggatgc gttagtcgat aagtctttcc aggaaaaatt
251   ccttcagtat tttcagtatt taaggataaa gcgaaggata accatatata
301   tacatatgta gtgaatagta tattaaatga gtattgacag cccaaactct
351   tttttctatg aaaaaagaaa gttaatagat tccatgttta agaacgaatc
401   gaatattcat tgatcaaatg attcactcca tagtctgata gatcttttca
451   cgaactgatt aatcagacga gaataaagat agagtcccat tttacatgtc
501   aattccggca acaatgaaat ttatagttaa gaggaaaatc cgtcgacttt
551   taaaatcgtg agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details