JRC GMO-Amplicons | EURL GMFF

Record ID:	OP133583.1
Database:	nt	Go to original record
Description:	Pentasachme caudatum voucher C.F. Zhang et al. 6056 chloroplast, complete genome.
Taxon:	Pentasachme caudatum
Original size:	158970 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50896	51459	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttcggaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcattggta gagaaacctt tccatcgaaa attcagaaag gctgaaagat
251   atacatacgt attgaatact atataaaatg attaatataa aatgattaat
301   gccgaccgaa atgagtctgt ttttttctat aaaaaacaga agaattggtg
351   tgattcgatt ctttcttcaa tgtggaatcg aatattcatt gatcaaaaga
401   ttcactccat agtctgtaga tcctcttttc aagaaccgga ttaatcggac
451   gagaataaag atagagtccc gttctacatg tcaatgctgg caacaatgca
501   attttgagta agaggaaaat ccgtcgactt caaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details