JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ459817.1
Database:	nt	Go to original record
Description:	Lundia spruceana voucher Lohmann 610 (SPF) plastid, partial genome.
Taxon:	Lundia spruceana
Original size:	157200 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46955	47520	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aaggaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcgttggta gaggaatctt tccattgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaatag attaatgatg
301   gcccgaatct gtatctgtat ttttttatat tatatgaaaa atggaagaat
351   tagtgtgaat tgattccaca ttgaagaaag aatcgaatat tcattcatca
401   aatcattcac tccatagtcc gatagttctt ttaaagaact gattaagcgg
451   acgagaataa agatagagtc ccattctaca tgtcaatgcc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttttaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details