JRC GMO-Amplicons | EURL GMFF

Record ID:	ON872373.1
Database:	nt	Go to original record
Description:	Gynostemma guangxiense chloroplast, complete genome.
Taxon:	Gynostemma guangxiense
Original size:	157590 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48916	49532	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaatca aataagggct
151   tcgaaagcga taaaaaaaag gggggtaggt gcagagactc aatggaagct
201   gttctaacaa aagagttgcc taggttgcgt tagtaaagga atgaatcctt
251   ccatcgaaac tccagaaaag atgaaagata aacgtatata cgtactgaaa
301   tactatttga ttaatgacga cccgaatctc tattttttat agttatctaa
351   aaaatgatat atatatatat ataaaaaacg aattgttatt aatcgattcc
401   aatatctcca agttgaaaaa agaatcgaat attcattgat caaatcattt
451   actccatcat agtctgatag atcttttgaa gaactgatta atcagatgag
501   aatagaataa agatagagtc ccattctaca tgtcaatacc gacaaaaatg
551   aaatttatag taagaggaaa atccgtcgac ttaaaaaaat cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details