JRC GMO-Amplicons | EURL GMFF

Record ID:	ON872372.1
Database:	nt	Go to original record
Description:	Gynostemma compressum chloroplast, complete genome.
Taxon:	Gynostemma compressum
Original size:	157419 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49124	49739	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaatca aataagggct
151   tcgaaagcga taaaaaaaaa ggggggtagg tgcagagact caatggaagc
201   tgttctaaca aatgagttgc ctaggttgcg ttagtaaagg aatgaatcct
251   tccatcgaaa ctccagaaaa gatgaaagat aaacgtatat acgtactgaa
301   atactatttg attaatgacg acccgaatct ctatttttta tagttatcta
351   aaaaatgata tatatatata taaaaaacga attgttatta atcgattcca
401   atatctccaa gttgaaaaaa gaatcgaata ttcattgatc aaatcattta
451   ctccatcata gtctgataga tcttttgaag aactgattaa tcagatgaga
501   atagaataaa gatagagtcc cattctacat gtcaataccg acaaaaatga
551   aatttatagt aagaggaaaa tccgtcgact taaaaaaatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details