JRC GMO-Amplicons | EURL GMFF

Record ID:	ON872371.1
Database:	nt	Go to original record
Description:	Gynostemma caulopterum chloroplast, complete genome.
Taxon:	Gynostemma caulopterum
Original size:	157767 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49173	49787	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaatca aataagggat
151   tcgaaagcga taaaaaaaag gggggtaggt gcagagactc aatggaagct
201   gttctaacaa atgagttgcc taggttgcgt tagtaaagga atgaatcctt
251   ccatcgaaac tccagaaaag atgaaagata aacgtatata cgtactgaaa
301   tactatttga ttaatgacga cccgaatctc tattttttat agttatctaa
351   aaaatgatat atatatatat aaaaaacgaa ttgttattaa tcgattccaa
401   tatctccaag ttgaaaaaag aatcgaatat tcattgatca aatcatttac
451   tccatcatag tctgatagat cttttgaaga actgattaat cagatgagaa
501   tagaataaag atagagtccc attctacatg tcaataccga caaaaatgaa
551   atttatagta agaggaaaat ccgtcgactt aaaaaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details