JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ286031.1
Database:	nt	Go to original record
Description:	Thladiantha nudiflora chloroplast, complete genome.
Taxon:	Thladiantha nudiflora
Original size:	156824 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50134	50754	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaaa aggtttcgaa
151   aacgagaaaa aaaggggtag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactacgttg cgttagtaaa ggaatgaatc cttccatcga
251   aactccagaa aagatgaaag ataaacgtat tacgtactca aatactattt
301   gagtaatgac gacccgaatc tctatttttt atatttttct ttttttttat
351   ttatataaat aatgatatat aaaaatgaac gaattcttat gaatcgattc
401   caatatatcc aagttgaaaa aagaatcgaa tattcattga tcaaatcatt
451   tactccagcc tacatagtct gatagatctt ttgaagaatt gattaatcag
501   atgcgaatag aataaagata gagtcccatt ctacatgtca ataccgacaa
551   aaatgaaatt tatagtaaga ggaaaatccg tcgactttta aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details