JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ101235.1
Database:	nt	Go to original record
Description:	Amana hejiaqingii isolate WMZ1487 chloroplast, complete genome.
Taxon:	Amana hejiaqingii
Original size:	151572 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45415	45979	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttttttttt caaaaaaaaa gataggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gagttgatta cgttacatgc
201   gctagtagcc ggaatccttc tatcaaaatt attgaaaaga tacccgccct
251   atatatgtag atatacatac tcacatagta aacgattgat taatcgcagc
301   ccaaatccat tatatatgaa aaatttaaga atcattgtga atctattcca
351   ttcgatattc caatcgaagt tggaggaaga atcgaatatt aaatgatcaa
401   attattcatt ccaaagtctg atagatcttt tgaaaaaccg attattcgaa
451   cgagaataaa gagagagtcc cattctacgt gtcaataccg acaacaatga
501   aatttatagt aaaaggaaaa tccgtcgact ttaaaagtcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details