JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ101218.1
Database:	nt	Go to original record
Description:	Amana baohuaensis isolate WMZ1425 chloroplast, complete genome.
Taxon:	Amana baohuaensis
Original size:	151492 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45366	45931	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttttttttt tcaaaaaaaa ggataggtgc
151   agagactcaa tggaagctgt tctaacaaat ggagttgatt acgttacatg
201   cgctagtagc cggaatcctt ctatcaaaat tattgaaaag atacccgccc
251   tatatatgta gatatacata ctcacatagt aaacgattga ttaatcgcag
301   cccaaatcca ttatatatga aaaatttaag aatcattgtg aatctattcc
351   attcgatatt ccaatcgaag ttggaggaag aatcgaatat taaatgatca
401   aattattcat tccaaagtct gatagatctt ttgaaaaacc gattattcga
451   acgagaataa agagagagtc ccattctacg tgtcaatacc gacaacaatg
501   aaatttatag taaaaggaaa atccgtcgac tttaaaagtc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details