JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ101215.1
Database:	nt	Go to original record
Description:	Amana wanzhensis isolate WMZ1412 chloroplast, complete genome.
Taxon:	Amana wanzhensis
Original size:	151258 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45110	45673	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttaaaaaaa aaaaaaaagg ataggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgattac gttacatgcg
201   ctagtagccg gaatccttct atcaaaatta ttgaaaagat acccgcccta
251   tatatgtaga tatacatact cacatagtaa acgattgatt aatcgcagcc
301   caaatccatt atatatgaaa aatttaagaa tcattgtgaa tctattccat
351   tcgatattcc aatcgaagtt ggaggaagaa tcgaatatta aatgatcaaa
401   ttattcattc caaagtctga tagatctttt gaaaaaccga ttattcgaac
451   gagaataaag agagagtccc attctacgtg tcaataccga caacaatgaa
501   atttatagta aaaggaaaat ccgtcgactt taaaagtcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details