JRC GMO-Amplicons | EURL GMFF

Record ID:	OM809718.1
Database:	nt	Go to original record
Description:	Tetraena hamiensis var. qatarensis chloroplast, complete genome.
Taxon:	Tetraena hamiensis var. qatarensis
Original size:	106720 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47346	47877	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttctg aaaaaaaaaa atgaattcaa
151   tcaaaagaaa aagaatcaaa aaaggatagg tgcagagact caatggaagc
201   tgttctaaaa aatggagttg actgctgcat tatgttagtc aaggaatcct
251   tgcatcaaaa ctttcaaaaa gacgaaggat aaactttctt tctataaaaa
301   tctaaaaaga aaaatacttt ttcaatcaat tggaagttga ataaagaatc
351   aaatataatt tgatcaaatc atttactcca aagtctgata tatctttttc
401   aaaatggatt agtcagatga gaataaagat agagtcccat tctatatgtc
451   agtatcgaca acaatgaaat ttatagtgag aggaaaatcc gtcgatttta
501   taaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details