JRC GMO-Amplicons | EURL GMFF

Record ID:	KT281152.1
Database:	nt	Go to original record
Description:	Alloteropsis semialata isolate LO2O chloroplast, complete genome.
Taxon:	Alloteropsis semialata
Original size:	139641 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48313	48921	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaataagt ggttgtcaaa
151   ctaaaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtgtaact ccctggaaat
251   tagagaaaga agggcacatc taatacacac gtatagatac tgacatagca
301   aacgattaat cacggaaccc atatcctaat ataggttctt ttttttattt
351   tttagaatga aattaggaat gattatgaaa tagaaaattc tgaatttttt
401   tagaattatt gtgaatccat tccaatcgaa tattgagtaa tcaaatcctt
451   caattcattg ttttcgagat ctttaaaaaa gtggattaat cgaacgagga
501   taaagagaga gtcccattct acatgtcaat actgacaaca atgaaatttc
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details