JRC GMO-Amplicons | EURL GMFF

Record ID:	HG008943.1
Database:	nt	Go to original record
Description:	Myrtus communis var. communis chloroplast partial tRNA-Leu gene, clone 30
Taxon:	Myrtus communis var. communis
Original size:	577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	577	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaatgg attgagcctt ggtatggaac
51    cctactaagt gccaactttc aaattcagag aacccctgga attaaagatg
101   ggcaatcctg agccaaatcc tgttttacga aaaccaacaa aacaataagg
151   gttcataaag cgagaataaa aaaggatagg tgcagagact caatgggagc
201   tgttctaaca aagggagttt acacgttgcg ttggtaaagg aatctttttc
251   tcgaaactcg agaaagggaa aggataaacc tatatacata cgtatacgta
301   ctgaatacta tctcaaatgc ttaatgacga cgcgaatttt ttaaaaggaa
351   agaattgttg tgaatcagaa tcaattctaa gttgaagaaa gagtcgaata
401   ttcattgatc aaatcattta ctccatggtc tgataaatct tttgaagaac
451   tgattaatcg gacgagaata aagatagagt cccattctac atgtcaatat
501   cgacaaaaat gaaatttata gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details