JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427949.1
Database:	nt	Go to original record
Description:	Trichosanthes tricuspidata isolate PEBO4048 chloroplast, complete genome.
Taxon:	Trichosanthes tricuspidata
Original size:	157452 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49739	50327	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaaaagggta
151   ggtgcagaga ctcaatggaa gctgttctaa caaatggagt tgactacgtt
201   gcgttagtaa aggaatgaat ccttctatcg aaactccaga aaagatgaaa
251   gataaacgta ttacgtactg aaatactatt tgattaatga caacccgaat
301   ctctattttt ttttatataa atataaaaaa tgatatataa aaatgaacga
351   attgttctga atcgattcca atatctccaa gttgaaaaaa gaatcgaata
401   ttcattgatc aaatcattta ctccatcata catagtctga tagatctttt
451   gaagaactga ttaatcagat gcgaatagaa taaagataga gtcccattct
501   acatgtcaat accgacaaaa atgaaattta tagtaagagg aaaatccgtc
551   gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details