JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427946.1
Database:	nt	Go to original record
Description:	Trichosanthes sp. PEBO4545 chloroplast, complete genome.
Taxon:	Trichosanthes sp. PEBO4545
Original size:	156405 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49664	50249	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaggg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcggtag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaaccca aatctctatt
301   tttttttttt tatataaata tcaaaaatga tatataaaaa tgaacgaagt
351   gttctgaatc gattccaata tctccaagtt gaaaaaagaa tcgaatattc
401   attgatcaaa tcatttactc catcatacat agtctgatag atcttttgaa
451   gaactgatta atcagatgcg aatagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaaaaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details